Baculovirus Expression Protocols

Abstract

Introduction to the Molecular Biology of Baculoviruses. Baculovirus Transfer Vectors. Insect Cell-Culture Techniques in Serum-Containing Medium. Insect Cell Culture in Serum-Free Medium. Transfection Techniques for Producing Recombinant Baculoviruses. Selection of Recombinant Baculoviruses by Visual Screening. Production of Recombinant Baculoviruses Using Linearized Viral DNA. Rapid Procedures for the Isolation and PCR Analysis of Recombinant Baculovirus. Production of Recombinant Baculoviruses Using Rapid Screening Vectors That Contain the Gene for b-Galactosidase. Fast Isolation of Recombinant Baculovirus by Antibody Screening. Continuous Foreign Gene Expression in Transformed Lepidopteran Insect Cells. Scale-up pf Recombinant Virus and Protein Production in Stirred-Tank Reactors. Expression of Foreign Genes in Bombyx mori Larvae Using Baculovirus Vectors. Expression of Foreign Proteins in Trichoplusia ni Larvae. Use of Baculoviruses as Biological Insecticides. Molecular Approaches to AIDS Vaccine Development Using Baculovirus Expression Vectors. Expression of the Hemagglutinin Protein of Influenza Virus: Analysis of Protein Modifications. Purification of Recombinant Protein Derived from the Baculovirus Expression System Using Glutathione Affinity Agarose. Purification of the Extracellular Domain of the Epidermal Growth Factor Receptor Produced by Recombinant Baculovirus-Infected Insect Cells in a 10-Liter Reactor. Expression and Secretion of a Soluble Form of Myelin-Associated Glycoprotein (MAG). Comparison of Different Cell Lines for the Production of Recombinant Baculovirus Proteins. Development of Lepidopteran Cell Lines. Index.