Abstract
Objectives: Bacteriorhodopsin is a heptahelical membrane protein found in the archaean Halobacterium salinarum. The performance of bacteriorhodopsin film was evaluated as a holographic optical memory. Material and method: Bacteriorhodopsin was immobilized on polymeric films with different weight/volume ratios of polyvinyl alcohol and gelatin. The effect of different concentrations of phosphate buffer (from 0.001 to 0.1 M) on performance of the polyvinyl alcohol / gelatin composite films was studied. Results: The results indicate that the best quality of the film was obtained in 0.001 % (w/v) of polyvinyl alcohol and gelatin mixture and 0.1 M phosphate buffer. For the control, bovine serum albumin was immobilized on polyvinyl alcohol/gelatin films instead of bacteriorhodopsin. The films based on bacteriorhodopsin were illuminated by two orthogonal beams, green laser (vertical) and red laser (horizontal) for 10 minutes, then their absorbance spectrum was measured. The green and red lasers were irradiated to the bacteriorhodopsin film for writing data which lead to absorption peaks about 380 nm (Q state) and 640 nm (O state). Finally, the decrease in absorption at 380 nm was monitored after 15 minute radiation of 1 watt blue LED utilized to erase the data. Conclusion: As a conclusion, this matrix is suitable for immobilization of bacteriorhodopsin protein and can be used in optical memory devices.
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