Abstract

Two avirulent clones of Agrobacterium tumefaciens which appeared spontaneously during culture are indistinguishable from the parental strains from which they were derived with respect to either sensitivity to the bacteriophages tested or the production of phages during growth. One attenuated clone, isolated after treatment of the virulent B6 strain with proflavin is able to induce limited proliferations in infected tissues that do not progress into visible tumors. These proliferations do not respond to exogenous auxin. Tumors appearing in decapitated pea seedlings inoculated with the attenuated clone after infection by virulent bacteria are larger than those of seedlings inoculated only with virulent bacteria. When introduced into tissues before virulent bacteria, attenuated bacteria inhibit tumor formation. Like the parental strain, the attenuated clone is lysogenic for phages inducible by ultraviolet irradiation and also by conditions within host tissues.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.