Abstract
Two avirulent clones of Agrobacterium tumefaciens which appeared spontaneously during culture are indistinguishable from the parental strains from which they were derived with respect to either sensitivity to the bacteriophages tested or the production of phages during growth. One attenuated clone, isolated after treatment of the virulent B6 strain with proflavin is able to induce limited proliferations in infected tissues that do not progress into visible tumors. These proliferations do not respond to exogenous auxin. Tumors appearing in decapitated pea seedlings inoculated with the attenuated clone after infection by virulent bacteria are larger than those of seedlings inoculated only with virulent bacteria. When introduced into tissues before virulent bacteria, attenuated bacteria inhibit tumor formation. Like the parental strain, the attenuated clone is lysogenic for phages inducible by ultraviolet irradiation and also by conditions within host tissues.
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