Abstract
Bacteriophage T7 DNA replication. An electron microscopic study of the growing point and the role of the T7 gene 4 protein in the formation of DNA fragments.
Highlights
T7 gene 4 codes for a protein whose function is essential for the replication of the viral chromosome
Analysis of the fine structure of the DNA growing points revealed a normal pattern: more than 90% of the replicating chromosomes contained a region of single-stranded template DNA on one side of the growing point
We have studied the nature of the events that occur in the T7 DNA growing point
Summary
T7 gene 4 codes for a protein whose function is essential for the replication of the viral chromosome. The experiments reported in this paper were designed to study the mechanism of action of T7 gene 4 by assessing the change that occurs in intracellular viral DNA when the gene 4 protein is inactivated during a normal DNA replication cycle. A straightforward interpretation of the data is that, upon the shift to 42”C, the T7 gene 4 protein is inactivated and DNA synthesis can occur on only one side of the growing point. In the absence of functional gene 4 protein, no new fragments can be developed to convert the region of single-stranded template DNA to the duplex state. Our in uiuo results form the complement for the in vitro studies of Scherzinger and Richardson and their colleagues, which demonstrate that purified gene 4 protein is capable of priming the de nouo initiation of DNA fragments
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