Abstract
This work describes a microfluidic cell sorting device that uses magnetophoresis to detect, separate, and concentrate tagged Listeria monocytogenes from food samples. Bacteriophage-functionalized magnetic particles were used to tag the bacteria in a food sample for pre-enrichment, which allowed for more rapid and selective cell sorting. We report on the effect of flow rate on the sorting efficiency, as well as the effect of two different channel designs on the sorting efficiency and purity of tagged bacteria within the food matrices of milk and ground beef. These enriched bacterial samples will be used in various biosensor applications for detection and identification of pathogenic bacteria in food samples.
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