Abstract
Urban activities generate surface deposits over impervious surfaces that can represent ecological and health hazards. Bacteriome genetic structures of deposits washed off during rainfall events, over an urban industrial watershed, were inferred from 16 S rRNA gene (rrs) sequences generated by high throughput sequencing. Deposits were sampled over a 4 year-period from a detention basin (DB). Major shifts, matching key management practices, in the structure of these urban bacteriomes, were recorded. Correlation analyses of rrs similarities between samples and their respective concentrations in chemical pollutants, markers of human fecal contaminations (HF183) and antimicrobial resistances (integrons), were performed. Harsher environmental constraints building up in the older deposits led to an increase number of rrs reads from extremophiles such as Acidibacter and Haliangium. Deposits accumulating in the decantation pit of the DB showed an increase in rrs reads from warm blooded intestinal tract bacteria such as Bacteroides and Prevotella. This enrichment matched higher concentrations of Bacteroides HF183 genotypes normally restricted to humans. Bacteriomes of urban deposits appeared good indicators of human-driven environmental changes. Their composition was found representative of their origin. Soil particles and rain appeared to be major contributors of the inferred bacterial taxa recovered from recent deposits.
Highlights
Cities are divided into patches favoring commercial, industrial, residential, and recreational activities
The above observations led us to hypothesize that urban deposits from a catchment should (1) harbor native soil microbial taxa and exogenous ones representative of the human activities occurring over an urban area; (2) have selected microbial taxa adapted for a use or detoxification of urban chemical pollutants; (3) have selected microbiota adapted for a survival and development in deposits that have settled among detention basins, and been exposed to strong environmental constraints including hydrological forces
Soil particles and rain appeared major sources of the bacterial taxa recovered among recent urban deposits investigated in this work
Summary
Cities are divided into patches favoring commercial, industrial, residential, and recreational activities. The above observations led us to hypothesize that urban deposits from a catchment should (1) harbor native soil microbial taxa and exogenous ones representative of the human activities occurring over an urban area; (2) have selected microbial taxa adapted for a use or detoxification of urban chemical pollutants; (3) have selected microbiota adapted for a survival and development in deposits that have settled among detention basins, and been exposed to strong environmental constraints including hydrological forces To address these issues, a Generation Sequencing (NGS) 16 S rRNA gene (rrs) analysis of accumulated urban deposits among a detention basin was performed. The NGS rrs genetic structures were compared between sampling sites, sampling years, and correlated to site maintenance practices, and hydrological and physico-chemical datasets
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