Bacteriological Study of Pseudomonas Aeruginosa Isolated from Different Infections and Study Antimicrobial Activities of Plant Extract Solanum Nigrum Against It

Abstract

This study was conducted for the period from 1/9/2016 to 30/3/2017 in Baquba city in Iraq. Eighty nine samples were collected from different infections from Baquba General Hospital and Al-Batool Hospital. Fifteen isolates (16.85%) were found to be Pseudomonas aeruginosa by using biochemical test. The bacterial diagnosis of all these isolates (100%) were confirmed by VITEK2, beside its possession of the 16s rDNA gene as determined by molecular detection. The results of virulence factors that had Pseudomonas aeruginosa showed possession of all isolates many virulence factors and a high production of which increases the pathogenicity of it. All isolates were able to produce hemolysin (100%), protease (73.33%), and biofilm formation (52%). The susceptibility test was applied on these isolates against (10) antibiotics. The results revealed that the highest resistances were for Ampicillin and Cefotaxime with 100% for each, while the lowest resistance were for Impineme (0.0%) and Ciprofloxacin (6.66%). The effects of ethanolic extract of fruits (Solanum nigrum) against P.aerugionsa were studied. At a concentration of 6% mg/ml, Solanum nigrum caused a marked increase in zone of inhibition (mm) on this bacteria growth. Inhibition zones sizes were different and increased according to concentration of extract and again the growth was completely inhibited in the highest concentration.