Bacteriological profile of wound infection in rural hospital in R.R District

Abstract

Background: Wound infection is a major problem in hospitals in developing countries. Wound infection causes morbidity and prolonged hospital stay thus the study was undertaken to know the bacteriological profile of wound infection and antibiogram of organisms isolated. Methods: pus from various sites of wound area was collected using sterile swab under aseptic precautions and further processed by Grams stain and culture. Culture was done on Blood agar, Mac Conkeys agar, incubated at 37°c for 24hrs.Isolates confirmed by biochemical tests, antibiotic susceptibility testing was done using muller Hinton agar by Kirby Bauer’s method as per standard CLSI guidelines. Results: out of 396 pus samples, 236 were culture positive. Most common organisms isolated was staphylococcus aureus 81 (34.3%) followed by Staph.epidermidis. Other isolates included were Enterococci, E.coli, Klebsiella, Pseudomonas, and Proteus spp. Conclusion: Present study showed bacteria causing wound infection. High rates of bacterial growth were seen in samples collected from surgery wards followed by orthopedics. S.aureus (34.3%) was the predominant isolated followed by CoNS (15.8%), E.coli (10.5%) MRSA incidence in our study was 10% and ESBL’s detected in 20% of E.coli strains and 6.6% Klebsiella strains.