Abstract
Pyogenic infections can be caused by various microorganisms and mixed infections are common which require antibiotic therapy. The inappropriate use of antibiotics has resulted in development of antibiotic resistance. The bacteriological profile may remain same, but antibiotic susceptibility pattern varies. Hence the study was conducted to know bacteriological profile of pus samples and their antibiotic susceptibility pattern. A retrospective study was carried out from January to June 2019. 108 pus samples collected during study period were included. The samples were cultured on Blood and MacConkey agar. After aerobic incubation at 37C for 18-24 hrs, organisms were identified by standard methods and antibiotic susceptibility was tested by Kirby Bauer disc diffusion method. ESBL was detected by combined disk test and Methicillin resistant Staphylococcus aureus (MRSA) by Cefoxitin 30 μg disc. Of 118 pus samples collected, 101(85.5%) were positive cultures and no growth was in 17(14.4%) samples. S. aureus 27(22.9%) was most common Gram positive isolate and Pseudomonas spp. 17(14.4%), was most common gram negative isolate. ESBL positivity was seen in 38(61.2%) and MRSA in 13(48.1%) S. aureus isolates. Most of gram negative isolates were susceptible to piperacillin / tazobactum 55(88.7%) and meropenem 53(85.4%) and gram positive isolates to vancomycin 27(100%) and linezolid 25(92.5%). The spread of beta-lactamase producing organisms has been increasing. Our study showed increased resistance to beta-lactam antibiotics which is a serious problem. To combat resistance irrational use of antibiotics should be avoided. Also regular surveillance helps in implementing better therapeutic strategies to reduce morbidity and mortality.
Highlights
Pyogenic infections can be caused by various microorganisms and mixed infections are common which require antibiotic therapy
S. aureus 27(22.9%) was most common isolate followed by Pseudomonas spp. 17(14.4%), E. coli 16(13.6%), CONS 10(8.5%), Citrobacter spp. 9(7.6%), Klebsiella pneumoniae and Enterobacter spp. 6(05.1%) each, Proteus spp. 5(04.2%), Acinetobacter spp. 3(02.5%) and Enterococcus spp. 2(1.7%)
ESBL positivity was seen in 38(61.2%) Gram negative isolates and most were susceptible to piperacillin / tazobactum 55(88.7%) and meropenem 53(85.4%)
Summary
Pyogenic infections can be caused by various microorganisms and mixed infections are common which require antibiotic therapy. The study was conducted to know bacteriological profile of pus samples and their antibiotic susceptibility pattern. Pyogenic infections refer to infection that causes pus formation and are characterized by several local inflammations, usually multiplication of microorganism.[1] Pus is a collection of thick, white or yellow fluid, formed at the site of inflammation during infection. It is made up of dead tissue, white blood cells, and damaged cells. Escherichia coli, Klebsiella sp., Proteus sp. and Enterococci sp. are commonly associated with pyogenic infections. 7,8
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