Abstract
INTRODUCTION: Bacteremia, is regarded as a significant reason in all over the world for morbidity and mortality in mainly sick patients. Invasion by microorganisms in blood constitutes the critical issues in infectious disease. The aims and objectives are to find out the pathogenic agents infecting males and females in blood of different age groups and naturally used antimicrobial sensitivity profiles. METHODS: All the blood samples were collected aseptically and tested in Popular Diagnostic Centre, Dhaka, Bangladesh between May, 2020 and October, 2020. Total 305 blood specimens from both sexes of different age groups were collected. Cultural analysis was performed by Gram staining, phenotypic examination, biochemical and serological analysis. Finally, antibiotic susceptibility test was done against commonly used antibiotics. RESULTS: 96 (31.47%) samples yielded a positive culture with predominant isolate being Salmonella typhi (41.66%) though Salmonella spp., Salmonella typhi, Staphylococcus spp and Pseudomonas spp. were also detected. Like most of previous reports, Salmonella spp was predominant, this corroborates this study. But the profile of antimicrobial susceptibility of the detected organisms varied comparing studies which were done in the past. The isolates were found mostly resistant to nalidixic acid. Most of the pathogens showed tremendous susceptibility against ceftriaxone, cefixime, ceftazidime etc. CONCLUSION: The antibiotic selection for the treatment of bacteraemia in patients should always be serious concern due to multidrug resistant (MRD) bacterial isolates. For proper treatment of anti-bacterial resistance and critical mortality and morbidity should be related with the sickness. For validating more reliability, this research requires further work.
Highlights
Bacteremia, is regarded as a significant reason in all over the world Received: March 16, 2021 for morbidity and mortality in mainly sick patients
Invasion by microorganisms in blood constitutes the critical issues in infectious disease
In the conclusion, we have performed blood culture by automated BD BACTECTMTM- 40 and in vitro analysis of antibiogram patterns from the patients who were suffering from acute febrile illness sought treatment at tertiary level hospital in Bangladesh
Summary
Blood is the connective tissue and indispensable for survival and proper functioning of human life. For common bacterial isolates finding, blood culture techniques are used. Development has been occurred in the recent years in blood culture media, culture techniques in the frameworks which are completely machine driven [4]. This research was done to find out the bacterial profile in blood culture and anti-biogram resistance profile of the isolated pathogens. The vials which gave a positive result were sub-cultured on Blood agar and MacConkey agar followed by CLSI routine microbiological techniques [6]. Descriptive statistics and chi-square tests were done for checking the statistical evaluation.
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