Bacteriological assessment of some borehole water samples in Mile 50, Abakaliki, Ebonyi State, Nigeria

Abstract

Water as excellent natural resource is meant to be of high quality to reduce the outbreak of water-borne diseases. Bacteriological load of some borehole water samples in Mile 50 Abakaliki were carried out to determine their potability. Fifteen borehole water samples were sampled during rainy and dry season from June to July and November to December 2018 respectively. The total bacterial count was determined by tenfold serial dilution method using peptone water. Eight bacterial species namely Vibrio cholerae, Pseudomonas aeroginosa, Escherichia coli, Staphylococcus aureus, Salmonella typhi, Shigella flexineri, Proteus vulgaris and Klebsiella pneumoniaewere isolated using standard analytical procedures. The bacterium that had the highest frequency of occurrence during both rainy and dry season’s was K. pneumoniae with percentage frequency of 21.81% and 20.79% respectively, and P. vulgaris had the least value of 6.96% during rainy season. E. coli and S. aureus have the least value of 5.94% during dry season. Amoxicillin (30ug) was mostly resisted by the bacterial isolates why being was more susceptible to Ciprofloxacin (10ug) among the antibiotics used for susceptibility test. Two way analysis of variance (ANOVA) was used to determine the level of significance among the bacteriological analyses of both seasons. Therefore, there is need to create awareness about the present situation of the borehole waters and the necessity for further treatment of water by consumers, before it can be used for both drinking and domestic purposes to prevent disease outbreak in the area.