Bacteriological and molecular study of Pseudomonas aeruginosa strains isolated from different clinical cases in Erbil and Kurkuk

Asif Hasan Abdul Razzaq

doi:10.30539/iraqijvm.v41i2.61

Abstract

This study included isolates of bacteria from 125 clinical samples in Erbil and Kirkuk Hospital including (burns, wounds, urine and sputum); 38 isolates were identified as P. aeruginosa after conducting microscopic and biochemical tests. The results of antibiotic sensitivity test showed that all isolates of P. aeruginosa were different in resistance to Pipracillin, Erythromycin with rate of (100%) and to the Nalidixic acid (94.73%) while the lowest resistant antibiotics were to Co-trimoxazole, Ceftazidime and Ciprofloxacin, which amounted to (26.31%, 23.68 and 21.05%) respectively. For molecular diagnosis of P. aeruginosa some virulence genes the alg D and exo A were amplified through Polymerase Chain Reaction technique. The results showed that in 38 isolates cases only 22 (57.9%) were positive for algD gene by amplification of 520 bp band. While in urinary tract infection; 6 samples (60%) had alg D gene, and 8 (57.14%) isolates had alg D gene in wounds samples; also 7(70%) isolates from burns had that gene, while the sputum samples showed only one with alg D gene which was the lowest ratio; but in amplification of exo A, the results showed the presence of only one isolate from burns with molecular weight 396 bp with no appearance in others.

Highlights

The P. aeruginosa is a ubiquitous expression is related to the presence of an environmental Gram-negative microorganism, operon composed by many genes with the which is one of the most important activation processes being regulated by (5); these genes are very infections and causes a wide variety of serious important for the existence of the mucoid trait, infections in individuals with thermal burn, the algD being responsible for the expression mechanical extensive trauma, cancer, cystic of the alginate capsule (6)
It causes community have revealed that P. aeruginosa is able to acquired infections; produce as many as three distinct it is associated with pneumonia, exopolysaccharides, each of which is cystic fibrosis and skin lesions (3)
The highest isolation rate was among burns patients (40%), Polymerase Chain Reaction (PCR) conditions: The primers were prepared by melting of alg D and exo A with distilled water; the size of 636 microliter, the initiators were blended well and stored on ice to complete the interaction; PCR master mix reaction was prepared by using (AccuPower PCR PreMix Kit)*and (GoTaq® Green Master Mix from Promega, USA)* this master mix was carried out according to the instructions of the company as shown in (Table, 2)

Summary

Introduction

Introduction the pathogenesis ofP. aeruginosa in which theThe P. aeruginosa is a ubiquitous expression is related to the presence of an environmental Gram-negative microorganism, operon composed by many genes with the which is one of the most important activation processes being regulated by (algR, opportunistic bacteria in hospital-acquired algP, algB and algU) (5); these genes are very infections and causes a wide variety of serious important for the existence of the mucoid trait, infections in individuals with thermal burn, the algD being responsible for the expression mechanical extensive trauma, cancer, cystic of the alginate capsule (6). It causes community have revealed that P. aeruginosa is able to acquired (hospital-acquired) infections; produce as many as three distinct it is associated with pneumonia, exopolysaccharides, each of which is cystic fibrosis and skin lesions (3). Overexpression of the alginate aeruginosa infections remains as a persistent exopolysaccharide was first identified as being problem, primarily because of the natural resistance of the organism and its remarkable associated with P. aeruginosa mucoid isolates recovered from the lungs of chronically ability to acquire resistance to multiple infected cystic firosis patients, but rarely from antimicrobial agents by various mechanisms other types of infections (8). Alginate production plays a central role in from urinary and respiratory tract infections, P. aeruginosa is believed to be a major contributory factor to chronic lung infections, which could form biofilm and adhere to human mucin in the lower respiratory tract and urinary system (9)

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