Abstract
The study was performed on 175 random samples of fresh broiler chicken and chicken meat products viz: Chicken burger;chicken luncheon; chicken kofta and chicken sausage (35 for each), collected from different shops at Benha city, kaliobiagovernment. The sample were examined for detection the prevalence of Staphylococci with special reference to S. aureusand MRSA, beside the phenotypic characterization of the isolated S. aureus strains and detection of their virulence genesin them. The bacteriological examination revealed the isolation of 98 Staphylococcus species including 41 S.aureus; 54S. epidermidis and 3S. chromogenes. In addition, all 41 isolated S. aureus were coagulase positive strains, while, the otherisolated Staphylococcal strains (57) were coagulase negative ones. Moreover, the results of SET- RPLA test revealed that5 S. aureus strains out of 10 randomly examined strains were enterotoxigenic and classified according to type of toxininto (3A; 1 B & 1C). The sensitivity tests for the isolated S. aureus showed strains indicated high resistance to methicillinfollowed by oxacillin; Nalidixic acid; Ampicillin; Amoxicillin; Cefotaxime and Tobramycin, respectively. Meanwhile,they were highly sensitive to Gentamycin; Enrofloxacin; Norfloxacin; Lomefloxacin and Ciprofloxacin. PCR resultscleared that, femA; mecA and hlb virulence genes were detected in all 5 S. aureus studied strains. Meanwhile, ica A wasdetected in 4studied strains; enterotoxin A (sea) was detected in 2 studied strains chicken meat and chicken kofta samplesonly and leukocidin (pvl) virulence gene was detected in 1 studied strain of chicken meat sample
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