Abstract
BackgroundButenyl-spinosyn, produced by Saccharopolyspora pogona, is a promising biopesticide due to excellent insecticidal activity and broad pesticidal spectrum. Bacterioferritin (Bfr, encoded by bfr) regulates the storage and utilization of iron, which is essential for the growth and metabolism of microorganisms. However, the effect of Bfr on the growth and butenyl-spinosyn biosynthesis in S. pogona has not been explored.ResultsHere, we found that the storage of intracellular iron influenced butenyl-spinosyn biosynthesis and the stress resistance of S. pogona, which was regulated by Bfr. The overexpression of bfr increased the production of butenyl-spinosyn by 3.14-fold and enhanced the tolerance of S. pogona to iron toxicity and oxidative damage, while the knockout of bfr had the opposite effects. Based on the quantitative proteomics analysis and experimental verification, the inner mechanism of these phenomena was explored. Overexpression of bfr enhanced the iron storage capacity of the strain, which activated polyketide synthase genes and enhanced the supply of acyl-CoA precursors to improve butenyl-spinosyn biosynthesis. In addition, it induced the oxidative stress response to improve the stress resistance of S. pogona.ConclusionOur work reveals the role of Bfr in increasing the yield of butenyl-spinosyn and enhancing the stress resistance of S. pogona, and provides insights into its enhancement on secondary metabolism, which provides a reference for optimizing the production of secondary metabolites in actinomycetes.
Highlights
Butenyl-spinosyn, produced by Saccharopolyspora pogona, is a promising biopesticide due to excellent insecticidal activity and broad pesticidal spectrum
Iron affects the growth and biosynthesis of butenyl‐spinosyn in S. pogona To explore the effects of iron on growth and secondary metabolism in S. pogona, different concentrations of FeSO4 (0 μM, 20 μM, 100 μM and 5 mM) were added into synthetic fermentation medium (SFM), the strain density of S. pogona and butenyl-spinosyn production were subsequently measured
High performance liquid chromatography (HPLC) was used to detect the S. pogona fermentation broth extract samples to explore the production of butenyl-spinosyn in different iron concentration (Fig. 1b)
Summary
Butenyl-spinosyn, produced by Saccharopolyspora pogona, is a promising biopesticide due to excellent insecticidal activity and broad pesticidal spectrum. Soil microbial actinomycetes can produce a variety of secondary metabolites, many of which are biologically active natural products that have important application value in industry, medicine and agriculture, and are valuable resources for human development and utilization [1,2,3]. Butenyl-spinosyn, a secondary metabolite produced by the aerobic fermentation of the soil actinomycete Saccharopolyspora pogona, is a spinosyn structural analog [4], that effectively kills pests by paralyzing the nervous system of insects [5].This unique insecticidal mechanism makes it powerful in the insecticidal spectrum, harmless to humans and animals, environmentally friendly, safe as a biological pesticide and as a chemical pesticide, giving it broad application prospects in agriculture [6, 7]. With the development of genetic manipulation technology and research on biosynthetic pathways and metabolic regulation networks, the use of genetic engineering, metabolic engineering, and construction of chassis cell heterologous biosynthesis has made strain modification easier and more efficient [12, 13]
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