Bacteriocin production by mucosal bacteria in current and previous colorectal neoplasia

Darina Kohoutova,Darina Kohoutova,Darina Kohoutova,Juraj Bosak,Jan Bures,Jan Bures,David Smajs,Jiri Cyrany,Stanislav Rejchrt,Stanislav Rejchrt,Miroslava Forstlova,Miroslava Forstlova,Paula Moravkova

doi:10.1186/s12885-020-6512-5

Darina Kohoutova, Darina Kohoutova + Show 11 more

Open Access

https://doi.org/10.1186/s12885-020-6512-5

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Abstract

BackgroundOptimal therapy for colorectal carcinoma (CRC), a frequently diagnosed malignancy, does not exist. Some of colicins and microcins, ribosomally synthesized peptides by gramnegative bacteria, have shown significant biological activity specifically against different cancer cells in vitro and in vivo conditions. The aim of this prospective study was to evaluate natural colicin and microcin production by large intestinal mucosal bacteria in each stage of colorectal neoplasia and in those with a history of colorectal neoplasia.MethodsA total of 21 patients with non-advanced adenoma (non-a-A; 16/21 with current and 5/21 with history of non-a-A), 20 patients with advanced colorectal adenoma (a-A; 11/20 with current and 9/20 with history of a-A), 22 individuals with CRC (9/22 with current and 13/22 with history of CRC) and 20 controls were enrolled. Mucosal biopsies from the caecum, transverse colon and the rectum were taken during colonoscopy in each individual. Microbiological culture followed. Production of colicins and microcins was evaluated by PCR methods.ResultsA total of 239 mucosal biopsies were taken. Production of colicins and microcins was significantly more frequent in individuals with non-a-A, a-A and CRC compared to controls. No significant difference in colicin and microcin production was found between patients with current and previous non-a-A, a-A and CRC. Significantly more frequent production of colicins was observed in men compared to women at the stage of colorectal carcinoma. A later onset of increased production of microcins during the adenoma-carcinoma sequence has been observed in males compared to females.ConclusionsStrains isolated from large intestinal mucosa in patients with colorectal neoplasia produce colicins and microcins more frequently compared to controls. Bacteriocin production does not differ between patients with current and previous colorectal neoplasia. Fundamental differences in bacteriocin production have been confirmed between males and females.

Highlights

Colorectal carcinoma (CRC) is after the lung and prostate cancer the third most frequently diagnosed malignancy in males and the second most common malignancy after the breast cancer in females
No significant difference in colicin and microcin production was found between patients with current and previous Non-advanced colorectal adenoma (non-a-A), between individuals with current and previous a-A, between patients with current and previous CRC; p>0.05
Fundamental differences in bacteriocin production have been confirmed between males and females

Summary

Introduction

Background Colorectal carcinoma (CRC) is after the lung and prostate cancer the third most frequently diagnosed malignancy in males and the second most common malignancy after the breast cancer in females. A total of 474,600 CRC related deaths in males and 387,100 in females were reported in 2018 worldwide [1]. Some of colicins and microcins, ribosomally synthesized peptides by gramnegative bacteria, have shown significant biological activity against different cancer cells in vitro and in vivo conditions. The aim of this prospective study was to evaluate natural colicin and microcin production by large intestinal mucosal bacteria in each stage of colorectal neoplasia and in those with a history of colorectal neoplasia

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Conclusion