Abstract

The retinal binding protein, bacterio-opsin, functions as a proton pump in the purple membrane of the extremely halophilic archaebacterium Halobacterium halobium. Under growth conditions of low oxygen tension and high light intensity, purple membrane synthesis is induced and H. halobium will grow phototrophically depending on the proton pump as the sole energy source. The bacterio-opsin gene (bop) is located within a cluster of genes which affect its expression. The bacterio-opsin activator gene (bat) appears to activate expression of the bop gene and the nearby bacterio-opsin related protein gene (brp). Transcription from these three genes is inducible by conditions of high light intensity and low oxygen tension.

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