Abstract
We conducted a comparative analysis of the impact of disinfecting preparations on the cryogenic stains of microorganisms, and also on Haemonchus contortus (Rudolphi 1803), invasive larvae of the ruminants. To test the preparations for disinfection, we used laboratory analyses with methods of biotesting, particularly with the use of Paramecium caudatum Her., Tetrahymena pyriformis Ehrenberg. We researched mixtures of substances: alkylbenzyldimethylammonium chloride (C24H42IN, BAK, mixture of homologues alkylbenzyldimethylammonium chloride and with n-C12H25, n-C14H29 and n-C16H33), didecyldimethylammonium Chloride (DDAC, C22H48ClN) and glutaraldehyde (C5H8O2); formaldehyde (CH2O), alkylbenzyldimethylammonium chloride and glutaraldehyde in 1% have bactericidal properties for the following cryogenic strains of microorganisms: Staphylococcus аureus, Salmonella typhimurium, Escherichia coli, Listeria monocytogenes, Proteus vulgaris, Serracia marcescens, Pseudomonas аeruginosa, Enterococcus faecalis and Yersinia enterocolitica. The Bacillus сereus were affected by the preparations bacteriostatically: we observed growth in the colonies in the medium with addition of 1% solution of mixture of alkylbenzyldimethylammonium chloride and didecyldimethylammonium chloride, and also 1%, 5% and 10% of solution of mixture of glutaraldehyde, formaldehyde and alkylbenzyldimethylammonium chloride. Also, these mixtures of substances have nematocidal properties. Death of 100% of L3 H. contortus after 24 hour exposure was observed with use of 1% solution of mixture of alkylbenzyldimethylammonium chloride and didecyldimethylammonium chloride, and also 5% glutaraldehyde, formaldehyde and alkylbenzyldimethylammonium chloride. Effective disinfection measures perform a leading role in providing stable veterinary well-being of livestock and healthcare of the population. Maximum toxicity during usage of the mixtures on P. caudatum was observed for the mixture of alkylbenzyldimethylammonium chloride and didecyldimethylammonium chloride, and also for formaldehyde and glutaraldehyde. The lowest toxicity for T. pyriformis was observed with use of the mixture of glutaraldehyde, sodium dodecylsulfate (SDS) and oleum terebinthini, and also the mixture of formaldehyde and glutaraldehyde, the highest formaldehyde and alkylbenzyldimethylammonium chloride. Thus, the most promising mixtures for use in veterinary medicine were determined to the following: alkylbenzyldimethylammonium chloride, didecyldimethylammonium chloride and glutaraldehyde, and also formaldehyde, alkylbenzyldimethylammonium chloride and glutaraldehyde.
Highlights
An obligatory component in the system of veterinary-sanitary measures for the objects of livestock farming is performance of disinfection
The mixtures we studied – alkylbenzyldimethylammonium chloride, didecyldimethyl ammonium chloride, glutaraldehyde, and alkylbenzyldimethylammonium chloride, formaldehyde, and glutaraldehyde – demonstrated bactericidal properties even in 1% concentration against cryogenic strains of the following microorganisms: S. аureus, S. typhimurium, E. coli, L. monocytogenes, P. vulgaris, S. marcencens, P. аeruginosa, E. faecalis and Y. enterocolitica. The mixtures of these substances demonstrated a bacteriostatic effect on B. сereus microorganisms: growth was observed in the colonies with addition of 1% of solution of mixture of alkylbenzyldimethylammonium chloride, didecyldimethyl ammonium chloride, and glutaraldehyde, and 1%, 5% and 10% solutions of mixture of alkylbenzyldimethylammonium chloride, formaldehyde, glutaraldehyde (Table 3)
The studies by Shirron et al (2009) allow us to state that didecyldimethyl ammonium chloride causes a bactericidal effect against S. typhimurium
Summary
An obligatory component in the system of veterinary-sanitary measures for the objects of livestock farming is performance of disinfection. A leading role in provision of stable veterinary well-being of livestock farming and healthcare of the population is played by the conducting of effective disinfecttion measures which cause the least possible harm to the environment. Disinfection preparations are tested using laboratory analyses with methods of biotesting, with ciliates. Correlation between the parameters of toxicity during comparative study of acute toxicity for the laboratory animals, ciliates б indicates that the ciliate T. pyriformis can be used an alternative model in predicting acute toxicity of pharmaceutical substances at the stage of their screening and pre-clinical study (Zhmin’ko et al, 2006)
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