Bactericidal Effect of Needle Plasma System on Pseudomonas aeruginosa

Abstract

Thirty Pseudomonas aeruginosa isolates identified using the API 20E system, biochemical and morphological characteristics then treated with needle plasma system (NPS) at different times. Pseudomonas aeruginosa isolates were tested by disk diffusion method to profile antibiotic susceptibility after and before NPS treatment. Our results of CFU show reduction in bacterial population by an average of (~ 3.1) log CFU from an initial (~ 23.3) log CFU. The percentage reduction (R%) was 100% which gave a very good indication of killing bacteria, for all isolates. The D-value was the average between 0.5 and 2.9 for all isolates according to treatment time 10–60 min. Antimicrobial sensitivity after NPS treatment has been increased. From this study, the NPS improved the high efficiency as physical bactericidal effects. The specific primer pairs (PA-SS forward, PA-SS reverse) were used to amplify (956) bp 16S rRNA region before and after NPS, then sequenced, the results of the locally clinical nosocomial bacteria isolates (query) were (99%) identical to 16S rRNA P. aeruginosa, and have no much difference in their 16S rRNA before and after PNS treatment but have an increase in the number of mutation after PNS: 2 G > T, 3,4 deletion G, 5 deletion C, 6 deletion A, 9,10 A > G, 13 G > T, 14 T > A, 17 deletion A, 21 T > G, 22 C > A, 23 A > T, 31 A > T, 34 C > G, 925, 926 deletion T, 927, 928 deletion A, 931 G > A.