Abstract
Aim. In this paper we investigate the impacts of co-incubation of Borrelia miyamotoi with neutrophils. Materials and methods. Spirochetes B. miyamotoi, strain HT31, were incubated 3 hours at 37°C with neutrophils of healthy donors (5*106 cells/ml) in a 1:1 ratio. The incubation medium contained also non-immune serum of healthy blood donors (SHD) and, in some experiments, high-immune serum of patients recovered from ITBB-BM (S-ITBB-BM). The proportion of neutrophils that bound borrelia, as well as the number and viability (mobility) of free borrelia, was estimated by dark-field microscopy. Results. Free-swimming borrelia remain viable in SHD or heat-inactivated S-ITBB-BM, but about 10% of borrelia are associated with neutrophils. In S-ITBB-BM with neutrophils, the proportion of viable borrelia among free ones decreases by approximately 10% compared to S-ITBB-BM without neutrophils; in addition about 15% of bor-relia become bound by neutrophils. If chemoattractant fMLP was added, the proportion of neutrophils binding borrelia increases to 25%, and the proportion of immobilized non-bound bor-relia reaches 40%. Conclusion. Although neutrophils are able to destroy borrelia with or without direct contact, under model conditions the combined effect of blood neutrophils and high-immune human serum does not provide 100% elimination of B. miyamotoi.
Highlights
ВЗАИМОДЕЙСТВИЕ НЕЙТРОФИЛОВ ЧЕЛОВЕКА С BORRELIA MIYAMOTOI, ВОЗБУДИТЕЛЕМ ИКСОДОВОГО КЛЕЩЕВОГО БОРРЕЛИОЗА
Olivieri R., Morandi M., Zanchi A. et al Evolution of macrolide resistance in Streptococcus pyogenes over 14 years in an area of central Italy
In this paper we investigate the impacts of co-incubation of Borrelia miyamotoi with neutrophils
Summary
ВЗАИМОДЕЙСТВИЕ НЕЙТРОФИЛОВ ЧЕЛОВЕКА С BORRELIA MIYAMOTOI, ВОЗБУДИТЕЛЕМ ИКСОДОВОГО КЛЕЩЕВОГО БОРРЕЛИОЗА Исследованы последствия инкубации Borrelia miyamotoi с нейтрофилами. В СЗД или С-ИКБ-БМ, инактивированной нагреванием, свободно плавающие боррелии остаются жизнеспособными, но около 10% боррелий оказывается связанными с нейтрофилами.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Journal of microbiology, epidemiology and immunobiology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.