Abstract
BackgroundIn recent years, the diode laser (810 nm) has been used for root canal disinfection, which plays an important role in endodontic therapy. This study was undertaken to evaluate the disinfecting ability of a diode laser in experimentally infected root canals of primary teeth.MethodsHuman retained mandibular primary anterior teeth without apical foramen resorption were selected and contaminated with Enterococcus faecalis for 21 days. The specimens were randomly divided into four groups: the negative group (no treatment), positive group (5.25% NaOCl), diode laser group (diode laser), and diode-NaOCl group (diode laser combined with NaOCl). The disinfecting abilities of the treatments were measured by the numbers of bacteria, scanning electron microscopy and confocal laser microscopy (live-dead staining).ResultsEighty teeth were selected. After irradiation and irrigation, the elimination of bacteria and the smear layer in the laser groups and positive group were significantly superior, compared with the negative group (p < 0.01). In the diode-NaOCl group, bacterial reduction reached nearly 100% on the surfaces of root canals; live bacteria were rarely observed, even in deeper dentinal tubules.ConclusionUse of a diode laser, especially in combination with NaOCl, was effective for disinfecting infected root canals of primary teeth.
Highlights
In recent years, the diode laser (810 nm) has been used for root canal disinfection, which plays an important role in endodontic therapy
This study evaluated the bactericidal efficacy of 810-nm diode laser irradiation and the combination of diode laser irradiation with 5.25% Sodium hypochlorite (NaOCl) in primary teeth by using
The root sections were incubated in brain-heart infusion (BHI) broth for 24 h at 37 °C to isolate them from bacterial contamination
Summary
The diode laser (810 nm) has been used for root canal disinfection, which plays an important role in endodontic therapy. The main objective of endodontic treatment is to effectively eliminate bacteria and necrotic pulp tissue remnants from the root canal system [1], in order to preserve the teeth. A biomechanical preparation cannot completely eliminate the microorganisms present in the Numerous studies have proven that the bactericidal effect of a diode laser (810 nm) is based on thermal properties; bacteria cannot develop resistance to laser exposure [5, 6]. Because of the complicated anatomical structure of the pulpal chamber [11], choosing the most effective disinfection protocol for pulp-infected primary teeth becomes important
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