Abstract
When we drink green tea infusion, we believe we are drinking the extract of the green tea leaves. While practically each tea bag infused in 300 mL water contains about 50 mg of suspended green tea leaf particles. What is the role of these particles in the green tea effect is the objective of this study. These particles (three different size ranges) were isolated via varying speed centrifugation and their respective inputs evaluated. Live oral bacterial samples from human volunteers have been screened against green tea extracts and macro, micro and nano sized green tea particles. The results showed that the presence/absence of the macro and mico sized tea particles in the green tea extract did not contribute much. However, the nano sized particles were characterized to be nature’s nano stores of the bioactive catechins. Eradication of these nano tea particles resulted in decrease in the bactericidal property of the green tea extracts. This is a curtain raiser investigation, busting the nano as well as green tea leaf particle contribution in green tea extracts.
Highlights
Several epidemiological studies as well as studies in animal models have confirmed the defense that green tea can offer, against various cancers such as those of the skin, breast, prostate and lung[19,20]
In order to optimize the concentration of the green tea extract for further experiments, six different concentrations (0 μ L, 50 μ L, 100 μ L, 200 μ L, 300 μ L and 500 μ L) of GT-0 and 5K-S from GT 1, GT 2 and GT 3 were tested against S. mutans
The most favorable effects of green tea are accredited to the green tea polyphenols, predominantly the catechins, which make up, 25–35% of the dry weight of green tea leaves[9,10,11]
Summary
Several epidemiological studies as well as studies in animal models have confirmed the defense that green tea can offer, against various cancers such as those of the skin, breast, prostate and lung[19,20]. The tea bags are after 3–4 min removed from the cup, or when using whole green leaves, the leaves are filtered using a sieve and the extract is consumed. This extract is the one that has been researched and whose potential widely published. The current work looks deep into this extract, which was observed to contain suspended particles from the green tea leaves. This work resolves this question by assessing one of the well established properties of green tea, which is antimicrobial activity, against oral microflora. The reason for choosing oral microflora is that this is the first place of contact of the green tea extract when consumed. The extracts rid of the particles were investigated for the first time
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