Abstract
Rabbits were vaccinated with the following Campylobacter fetus var. venerealis (Vibrio fetus) antigens: whole-cell (WC), autoclaved (A), boiled (B), and purified postgrowth broth (PGB). Bactericidal activity of freshly drawn heparinized blood against the organism was determined after each vaccination. In all cases bactericidal activity of the blood of vaccinated rabbits was higher than for nonvaccinated rabbits. The in vitro bactericidal activity of the blood was determined in two separate experiments. In experiment I the bactericidal activity of the blood of rabbits vaccinated with PGB antigen was the same as that of rabbits vaccinated with WC antigen and higher than that of rabbits vaccinated with A antigen after the third vaccination. In experiment II the bactericidal activity of blood of rabbits vaccinated with PGB antigen was the same as that of those vaccinated with WC antigen after the second and third vaccinations and higher than for rabbits vaccinated with A antigen after the third vaccination. Blood of rabbits vaccinated with A antigen was less bactericidal than blood of rabbits vaccinated with B antigen after the third vaccination, indicating the presence of a surface antigen destroyed by autoclaving but not by boiling. The in vivo and in vitro whole blood bactericidal tests are more sensitive for measuring the response of rabbits vaccinated with WC, B, A, or PGB antigens than is the plate agglutination test.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
