Bacterial-algae biofilm enhance MABR adapting a wider COD/N ratios wastewater: Performance and mechanism

Abstract

Although membrane aerated biofilm reactor (MABR) is promising in nitrogen removal due to its counter-diffusion biofilms structure, it still cannot adapt a wider COD/N ratios wastewater. In this condition, expanding the MABR applicability range in different COD/N ratio wastewater is necessary. In this study, a bacterial-algae biofilm, instead of bacteria biofilm, supporting membrane aerated biofilm reactor (MABAR) was constructed, and the performance was compared to MABR. Results showed that the total nitrogen (TN) removal efficiency was promoted significantly in MABAR regardless of the COD/N ratio. Compared to MABR, effluent TN concentration in COD/N ratio of 2, 5, and 8 declined by 14.34 mg/L, 0.50 mg/L, and 12.10 mg/L, respectively. Nitrification inhibition test suggested that algae assimilation made an obvious contribution (at least 18.18 mg/L) to the NH4+-N removal in MABAR. Besides, redundancy analysis (RDA) indicates that MABAR has a negative correlation with Nitrospirae but is positively correlated with NH4+-N removal load. These results are consistent with the kinetics result that algae assimilation, instead of nitrification-denitrification, is responsible for the nitrogen removal in MABAR. Therefore, the change of nitrogen removal route further gave MABAR excellent adaptability and impact resistance to address wastewater with different COD/N ratios, which is conducive to its wide application.