Abstract
The bacterial transformation of pyrene in an estuary environment, Kitimat Arm, British Columbia, Canada, was studied. A metabolite was separated from marine sediment and pore water samples and was identified as cis-4,5-dihydroxy-4,5-dihydropyrene, based on a comparison of the metabolite with the authentic standard, by using both high-performance liquid chromatography and capillary gas chromatography with mass spectrometry detection. The presence of this key metabolite of dioxygenase-mediated transformation of pyrene, along with previous pyrene degradation studies using cultures isolated from the same sediment samples, suggests a possible in situ bacterial transformation of pyrene in the Kitimat Arm environment.
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