Bacterial three-dimensional genome architecture

Abstract

Using a combination of chromosome conformation capture carbon copy (5C), three-dimensional modelling and live cell imaging, the authors were able to resolve the three-dimensional architecture of the Caulobacter crescentus bacterial genome. They show the genome to be ellipsoidal with periodically arranged chromosome arms. The location of parS sequence elements dictated the subcellular location of the genome; however, artificially altering this location had little effect on large-scale gene expression.