Abstract

Six bacterial isolates from the U.S. DOE Subsurface Science Program and three references bacteria were tested for resistance to UV light and gamma radiation. The subsurface isolates included three aerobic, pigmented, Gram positive bacteria and three microaerophilic, non-pigmented, Gram negative bacteria. Deinococcus radiodurans was the most resistant bacterium to both types of radiation, with a D 37 value of 4.0 × 10 4 μWs cm −2 to UV light and 300 krads to gamma radiation. The aerobic subsurface bacteria were found to be significantly more resistant ( p < 0.05) than the microaerophilic subsurface bacteria to UV light and gamma radiation. Due to the similarities of bacterial survival between UV and gamma radiation; it is proposed that UV light could be utilized to model the fate of microorganisms exposed to ionizing radiation.

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