Bacterial productivity and protistan bacterivory in coastal and offshore communities of Lake Erie

Abstract

The importance of protists as bacterivores in a coastal community and an offshore community of Lake Erie was compared during the summer of 1994. Bacterial density, cell size, and empirical conversion factors for bacterial productivity were highly variable at both sites and greater at the coastal site (P < 0.01). Bacterial productivity at the coastal site was 25-50 times higher than at the offshore site. Bacterivory was estimated in situ by fluorescently labeled native bacteria. Per-cell grazing rate and filtering rate for each taxon were routinely determined. Heterotrophic nanoflagellates (HNAN) were the most important protistan bacterivores at the offshore site, while HNAN and ciliates were similarly dominant bacterivores at the coastal site. Mixotrophic bacterivory was important only at the offshore site where Dinobryon was the dominant bacterivore. Bacterial carbon flux through protists was higher at the coastal site by an order of magnitude. Offshore protists grazed virtually the entire bacterial production, while coastal protists usually grazed less than half of the bacterial production. These results suggest that coastal and offshore sites differed fundamentally in the significance of protists to carbon flux through the microbial loop to higher trophic levels.