Abstract
Background and Aim:Anticoagulants with different modes of action are used in the collection of camel blood samples. In the innate immune response, camel neutrophils and monocytes can play several roles during infection and inflammation. For anticoagulants ethylenediaminetetraacetic acid (EDTA) and heparin, research has described their effects on different parameters of the immune system. However, to date, no research has examined the effects of anticoagulants on the functional activity of camel phagocytes. Therefore, this study analyzed the influence of K3EDTA and lithium heparin on the antimicrobial activity of camel neutrophils and monocytes.Materials and Methods:Camel leukocytes were separated from blood collected in EDTA or heparin tubes, and their phagocytosis and reactive oxygen species (ROS) production activity were analyzed by flow cytometry after stimulation with Staphylococcus aureus or Escherichia coli bacteria.Results:In comparison to the cells collected from the EDTA blood, the camel neutrophils and monocytes separated from the heparin blood showed higher phagocytosis activity of S. aureus and E. coli. In addition, the neutrophils and monocytes produced significantly more ROS when the blood was collected in the heparin tubes.Conclusion:The antimicrobial functions of camel neutrophils and monocytes are significantly affected by the type of anticoagulation agent. Therefore, using heparin rather than EDTA as an anticoagulant is recommended when performing the functional analysis of phagocytosis and ROS production of camel phagocytes.
Highlights
Camel (Camelus dromedarius) polymorphonuclear neutrophils and monocytes are key players in the immune system, having several roles during infection and inflammation [1]
In comparison to the cells collected from the ethylenediaminetetraacetic acid (EDTA) blood, the camel neutrophils and monocytes separated from the heparin blood showed higher phagocytosis activity of S. aureus and E. coli
The antimicrobial functions of camel neutrophils and monocytes are significantly affected by the type of anticoagulation agent
Summary
Camel (Camelus dromedarius) polymorphonuclear neutrophils and monocytes are key players in the immune system, having several roles during infection and inflammation [1]. For the collection of blood samples, anticoagulants with different modes of action have been used [4]. The heparin anticoagulation effect depends on preventing the conversion of fibrinogen into fibrin [5]. For both anticoagulants, several effects on cell phenotype and function have been described previously [6,7,8,9]. Anticoagulants with different modes of action are used in the collection of camel blood samples. For anticoagulants ethylenediaminetetraacetic acid (EDTA) and heparin, research has described their effects on different parameters of the immune system. To date, no research has examined the effects of anticoagulants on the functional activity of camel phagocytes. This study analyzed the influence of K3EDTA and lithium heparin on the antimicrobial activity of camel neutrophils and monocytes
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