Bacterial pathogens associated with the plastisphere of surgical face masks and their dispersion potential in the coastal marine environment

Abstract

Huge numbers of face masks (FMs) were discharged into the ocean during the coronavirus pandemic. These polymer-based artificial surfaces can support the growth of specific bacterial assemblages, pathogens being of particular concern. However, the potential risks from FM-associated pathogens in the marine environment remain poorly understood. Here, FMs were deployed in coastal seawater for two months. PacBio circular consensus sequencing of the full-length 16S rRNA was used for pathogen identification, providing enhanced taxonomic resolution. Selective enrichment of putative pathogens (e.g., Ralstonia pickettii) was found on FMs, which provided a new niche for these pathogens rarely detected in the surrounding seawater or the stone controls. The total relative abundance of the putative pathogens in FMs was higher than in seawater but lower than in the stone controls. FM exposure during the two months resulted in 3% weight loss and the release of considerable amounts of microfibers. The ecological assembly process of the putative FM-associated pathogens was less impacted by the dispersal limitation, indicating that FM-derived microplastics can serve as vectors of most pathogens for their regional transport. Our results indicate a possible ecological risk of FMs for marine organisms or humans in the coastal and potentially in the open ocean.