Abstract
Abstract The aim of this study was to find an arsenite-resistant bacterial strain, having high oxidizing capability from As(III) to As(V) in soil and stabilize the bio-available arsenic. An As(III) oxidizing bacteria strain XS3, was isolated from arsenic-rich gold mine tailings in Xinjiang, China. XS3 exhibits high resistance to As(III) and was able to oxidize up to 6,400 mg/L in sucrose minimal salt low phosphate (SLP) medium, higher than any of the previous reported Pseudomonads. To determine the molecular basis of resistance and transformation aoxB/aioA gene encoding arsenite oxidases were amplified using a degenerate primer. The aioA gene was specific for the arsenite-oxidizing bacteria, and deduced amino acid sequence revealed 62% homology with Pseudomonas arsenicoxydans. Arsenite oxidase enzyme activity from this isolate displayed a high arsenite resistance level. Based on 16S rRNA gene sequence analysis XS3 was closely related to Pseudomonas sp. Strain XS3 showed potential for soil remediation by r...
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