Abstract
AIM: To evaluate the microleakage at the implant-abutment (I-A) interface of Morse tapered implants inoculated with different volumes of bacterial suspension.METHODS: Morse tapered I-A sets were selected and divided in two groups depending on the type of abutment: passing screw (PS) and solid (S), and then subdivided into four subgroups (n=6) according to the suspension volume: PS1: 0.1 µL; PS3: 0.3 µL; PS5: 0.5 µL; PS7: 0.7 µL; S1: 0.1 µL; S3: 0.>3 µL; S5: 0.5 µL and S7: 0.7 µL. A control test was performed to verify the presence of external contamination during the inoculation and the implants were incubated for microbiological analysis. The microleakage was evaluated every 24 h for 7 days by the clarity of solution. After this period, the implants were disassembled for confirmation of bacterial viability.RESULTS: All the specimens with 0.7 µL and one sample of S5 presented turbidity in the control test indicating external contamination, and were excluded from the study. After 7 days of observation, none of the specimens presented positive results for microleakage and the bacterial viability was confirmed in all specimens. The 0.1 µL and 0.3 µL volumes did not present bacterial microleakage, meaning that these volumes may be inadequate for analysis.CONCLUSIONS: None of the sets evaluated showed bacterial microleakage at the I-A interface and the volume of 0.7 µL exceeded the internal capacity of the implants.
Highlights
Received for publication: February 20, 2014 Accepted: March 28, 2014Failures in implant therapy have been associated with lack of stability or misfit at the implant-abutment (I-A) interface[1]
Two-piece implants have a microgap depending on the interface type or system, but presence of fluid flow at this interface and its relationships are very variable . 2-18 This has been correlated to the presence of bacterial infiltration and inflammatory cells that may lead to bone loss around this area[19]
Zero CFUs of E. coli were detected from sampling of abutments that were maintained sterile (Figure 1A), whereas 10 McFarland (30 x 108 CFU/mL) of E. coli were detected in samples from abutments exposed to bacterial culture (Figure 1B)
Summary
Received for publication: February 20, 2014 Accepted: March 28, 2014Failures in implant therapy have been associated with lack of stability or misfit at the implant-abutment (I-A) interface[1]. 2-18 This has been correlated to the presence of bacterial infiltration and inflammatory cells that may lead to bone loss around this area[19]. Implant manufacturers try to reduce bacterial infiltration by increasing the accuracy and stability of the jointed pieces by fabricating very high precision mechanical parts 7. Internal conical joints have greater mechanical stability of the I-A interface and have shown no crest bone loss[20,21]. This has been explained by the stress distribution of the implant’s long axis during function. The greater stability of the soft tissues provided by the tapered abutment design and its reduced diameter relative to the platform and absence of abutment micromobility by the self-locking feature reduces bacterial leakage at the interface or even prevents it[3,4,5,6,7]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
