Abstract
Bone infections are common in trauma-induced open fractures with bone defects. Therefore, developing anti-infection scaffolds for repairing bone defects is desirable. This study develoepd novel Mg-based porous composite scaffolds with a basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium phosphate (TCP). A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion and biofilm formation of Staphylococcus aureus were evaluated. The results indicated that the Mg-based scaffolds significantly inhibited bacterial adhesion and biofilm formation compared to PT, and the PT10M and PT15M exhibited significantly stronger anti-biofilm ability than PT5M. In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg2+ concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds. Additionally, the PT15M exhibited an inhibitory effect on cell adhesion and proliferation of MC3T3-E1 cells. In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.
Highlights
Various bone substitutes have been developed for bone defect repair applications, including biodegradable polymers, bioceramics, and polymer-bioceramic composites
Biodegradable polymers, such as polylactic acid (PLA), polyglycolic acid (PGA), poly(lactic-co-glycolic acid) (PLGA), and poly-ε -caprolactone (PCL), can gradually be replaced by new bone, but their mechanical strength is too low[4], and their degradation products may result in some side effects[5]
We successfully fabricated a porous PLGA/tricalcium phosphate (TCP) scaffold using the Low-temperature deposition manufacturing (LDM) technique, and we demonstrated that the PLGA/TCP scaffold exhibited good bone-repairing ability and can be used as a drug carrier[38,39,40,41]
Summary
Various bone substitutes have been developed for bone defect repair applications, including biodegradable polymers, bioceramics, and polymer-bioceramic composites Biodegradable polymers, such as polylactic acid (PLA), polyglycolic acid (PGA), poly(lactic-co-glycolic acid) (PLGA), and poly-ε -caprolactone (PCL), can gradually be replaced by new bone, but their mechanical strength is too low[4], and their degradation products may result in some side effects[5]. The mechanism for the antibacterial ability of these Mg-based scaffolds was investigated
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