Bacterial Indicators and Environmental Factors as Related to Contamination of Oysters by Enteroviruses

Abstract

Enteric viruses previously have been reported in marine waters and shellfish which met acceptable bacteriological standards for recreational use and shellfish harvesting. Unfortunately, previous data on occurrence of enteric viruses are limited and usually not quantitative. In this report, results of studies conducted along the upper Texas Gulf coast, where a substantial amount of quantitative virological data was collected, are compared to bacteriological indicators and other environmental factors on a statistical basis. A product-moment correlation matrix showed that there was a moderate correlation between viruses in water and total coliforms in water, total coliforms in oysters and fecal coliforms in oysters. However, presence of viruses in water was not found to be correlated with presence of viruses in oysters. The only significant regression coefficient found for the model relating the concentration of viruses in water to bacterial indicators and other environmental variables was concentration of coliforms in oysters. Multiple regression analysis showed that approximately 25% of the variance in the number of viruses detected in water was statistically accounted for by the linear correlation with the total coliforms in oysters. The amount of variation in the number of viruses explained by this indicator, however, was not large enough to make the concentration of coliforms in oysters a good predictor of the concentration of viruses in water. Furthermore, none of the bacterial or other environmental variables was found to be a good predictor of the concentration of viruses detected in oysters. Our failure to find a strong predictive relationship between viruses in marine water and in oysters, and the occurrence of viruses in high frequency in waters which met current bacteriological standards, indicate that these standards do not reflect the occurrence of enteroviruses in marine waters.