Abstract
Ureolytic activity was detected in liver homogenates of two carcharhinid shark species, Galeocerdo cuvieri and Negaprion brevirostris, employing 14C-labeled urea to monitor mineralization over 48 h. Significant (P < 0.05) urea hydrolysis was detected in liver homogenates within 48 h whereas no hydrolysis was detected in homogenates incubated with either ampicillin or the vibriostatic agent O/129, indicating bacterial mediation of the ureolytic activity. Urea hydrolysis could not be detected in blood samples. The autochthonous microflora of sharks has been found to contain ureolytic bacteria, and the present study supports the conclusion that they are present in organ tissues of healthy sharks but are not present in the blood.
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More From: Canadian Journal of Fisheries and Aquatic Sciences
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