Abstract
SPECIFIC AIMSBacterial CpG DNA has been shown to activate immune cells through Toll-like receptor 9 (TLR-9). We sought to determine whether 1) enteric epithelial cell lines express TLR-9 and 2) exposure to Escherichia coli DNA would elicit an epithelial response as defined by mediator synthesis (i.e., IL-8, PGE2) or altered barrier or ion transport.PRINCIPAL FINDINGS1. Expression of TLR-9 mRNA in human colonic epithelial cellsThe human colonic HT-29, Caco-2, T84, and airway A549 epithelial cells were analyzed for the expression of TLR-9 by RT-PCR. THP-1 (human monocyte cell line) and CCD-18Co (human fibroblast cell line) cells served as positive and negative controls, respectively. All four epithelia constitutively expressed TLR-9 mRNA. Purification and sequencing of the PCR product from Caco-2 cells revealed a 100% match with the published human TLR-9 cDNA sequence. Demonstration of TLR-9 expression complements recent reports of other TLRs (e.g., TLR-4) on gut epithelia.2. E. coli DNA induces epithelial ...
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