Abstract
Little is known about extremophilic microorganisms from glaciers found in subtropical regions, and to our knowledge, no reports have identified glacial bacteria in this ecosystem in Mexico. Herein, we report a 16S rRNA gene amplicon data set demonstrating bacterial diversity of three samples from the Iztaccihuatl volcanic complex (Mexico) with a total of 115,701 to 138,805 high-quality reads. The bacterial population was classified at the phylum level in all samples.
Highlights
Little is known about extremophilic microorganisms from glaciers found in subtropical regions, and to our knowledge, no reports have identified glacial bacteria in this ecosystem in Mexico
Calvillo-Medina et al FIG 1 Bar chart of the bacterial diversity based on 16S rRNA gene amplicon analysis of samples from the Iztaccihuatl volcano
To identify operational taxonomic units (OTUs), 16S rRNA gene metagenomic libraries were clustered, and processed sequences were analyzed with the 16S biodiversity tool and classified with RDP tools version 2.12 [7], with 97% identity of bacterial diversity
Summary
Little is known about extremophilic microorganisms from glaciers found in subtropical regions, and to our knowledge, no reports have identified glacial bacteria in this ecosystem in Mexico. Glacier samples were collected in sterile glass containers of 500 ml by boring holes to a depth of 60 cm using a sterile ice axe (sterilized on site using 96° ethanol and flaming). Crater lake samples (200 ml) underneath the frozen lake surface were obtained by cracking the ice surface to create a hole using a sterile ice axe and submerging sterile glass containers to a depth of 60 cm.
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