Abstract
Lignocellulose pretreatment by ionic liquid (IL) displays enormous potential in biomass conversion, however, high cost impedes its large-scale application in biorefinerry process. In this study, a strategy evolving a high substrate loading was developed to address this challenge. Rice straw (RS) was first pretreated with 1-ethyl-3-methylimidazolium acetate at a high substrate loading of 25 wt.%, and then followed by bacterial delignification. The corresponding reducing sugar and glucose yield was 510.3 and 324.5 mg/g, respectively, which increased by 1.4 and 1.37 times over those of the sole IL pretreatment. The IL pretreatment significantly changed the structure of RS and reduced the crystallinity of cellulose, and the biological pretreatment with a bacterial strain of Cupriavidus basilensis B-8 removed most of the lignin residual. Meanwhile, the cellulose was well reserved. The combination pretreatment contributed to a high yield of the fermentable sugar in an economical way.
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