Abstract
Abstract—A bacterium, Novosphingobium sp. KKU03 previously demonstrated to degrade the cyanobacterial toxin, microcystins (MCs), was investigated for the removal of MCs through the slow sand filter. In this study, biological sand filtration was assessed in PVC column experiment for its ability to remove MCs ([Dha 7 ]MC-LR and MC-LR). Degradation of MCs was observed with inoculated (6x10 8 CFU/ml) treatment of water dosed with both MCs (completed degradation within 7 days) compared to uninoculated control. Polymerase chain reaction (PCR) specifically targeting amplification of 16S rRNA gene of Novosphingobium sp. KKU03 was applied to monitor the presence of the bacterium in experimental trials. PCR products indicative of an endemic bacterial population were observed at all sample sites through the column where MCs degradation was measured, indicating this bacterial isolate was active in degradation of MCs.
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