Bacterial community structure of mangrove sediments in relation to environmental variables accessed by 16S rRNA gene-denaturing gradient gel electrophoresis fingerprinting

Yanying Zhang,Youshao Wang,Si Zhang,Bin Yang,Juan Ling,Junde Dong

doi:10.3989/scimar.2009.73n3487

Yanying Zhang, Youshao Wang + Show 4 more

Open Access

https://doi.org/10.3989/scimar.2009.73n3487

Copy DOI

Abstract

Bacterial community structure and the relationship between environmental variables and microbial communities in the surface sediments of tropical mangrove ecosystems were investigated in Sanya, Hainan Island, China. Profiles of bacterial communities were generated using culture-independent PCR-denaturing gradient get electrophoresis (DGGE), and the results were interpreted with multivariate statistical analysis. Findings suggested that microbial communities varied with sample collection sites and seasons. The samples collected from the same sample sites tit the same time had more similar microbial communities except samples SH3 and AB5, which also had unique sediment quality. Canonical correspondence analysis (CCA) revealed that the organic carbon concentration of the sediments accounted for a significant amount of the variability in the bacterial community composition. Phylogenetic analysis was used to identify the major groups of the predominant bacterial phylotypes. 16S rRNA gene-V3 fragments from 17 individual DGGE bands were sequenced and the corresponding bacteria were found in mangrove sediments for the first time based on BLAST results. Predominant bacterial phylotypes Clustered with various taxonomic groups, including Proteobacteria, Bacteroidetes, Gemmatimonadetes, Actinobacteria and Firmicutes.

Highlights

Mangrove ecosystems are dominant ecosystems along tropical coastlines
SUMMARY: Bacterial community structure and the relationship between environmental variables and microbial communities in the surface sediments of tropical mangrove ecosystems were investigated in Sanya, Hainan Island, China
Profiles of bacterial communities were generated using culture-independent PCR-denaturing gradient gel electrophoresis (DGGE), and the results were interpreted with multivariate statistical analysis

Summary

Introduction

Mangrove ecosystems are dominant ecosystems along tropical coastlines They have important relationships with the regulation and optimisation of tropical marine environments. Knowledge of microbial community diversity and the relationship between environmental factors and members of bacterial communities in mangrove sediments is important for understanding how the mangrove ecosystems function; this is necessary to formulate effective management and conservation strategies. Molecular techniques based on culture-independent techniques such as denaturing gradient gel electrophoresis (DGGE), automated ribosomal intergenic spacer analysis (ARISA) and terminal restriction fragment length polymorphism (TRFLP) have provided new tools for genetically identifying bacteria in all kinds of environmental systems, which has enhanced the knowledge of the in situ population structure of bacteria communities (Li et al, 2006; Liang et al, 2007; Muckian et al, 2007). As DGGE can separate sequences of the same length with only a base difference, 16S rRNA gene-DGGE fingerprinting is useful as an initial investigation to compare communities and identify the qualitative or most relatively abundant community members (Muyzer et al, 1993)

Objectives

Methods

Results

Discussion

Conclusion