Bacterial Community Structure Characteristics in the Biliuhe Reservoir and Its Key Driving Factors

Abstract

In order to understand the bacterial community structure and its diversity in the Biliuhe Reservoir, the impact of environmental factors must be explored. Using 16S rDNA high-throughput sequencing technology, the bacterial community structure and diversity differences in four seasons were analyzed and compared from 2016 to 2017. Redundancy analysis (RDA) was used to investigate the relationship between the environmental factors in water and bacterial diversity. The analysis of bacterial diversity index (Shannon-Wiener index) showed that the bacterial community diversity index reached 6.9 in the whole year, with the following pattern:Winter < summer < spring < autumn. Sequence alignment results show that although the flora status was not clear and rare flora were seen, a total of 44 bacterial phyla, 115 classes, 184 orders, 368 families, and 865 genera were found. The dominant bacteria phyla were:Proteobacteria (34.16%), Cyanobacteria (24.35%), Actinobacteria (13.35%), Bacteroidetes (12.42%). The dominant class of bacteria in the dominant phylum (Proteobacteria) was β-Proteobacteria (18.34%), and the dominant strain was unidentified_Chloroplast (17.73%). The results of RDA showed that different bacterial communities were affected by environmental factors. Total suspended solids (TSS), transparency (SD), pH, active phosphorus (ADP), dissolved oxygen (DO), total phosphorus (TP), and nitrite nitrogen (NO2--N) were the main environmental factors influencing community distribution. The bacterial community during the summer was positively correlated with NO2--N, ADP, TP, and TSS.