Bacterial communities in sediments of Lake Baikal from areas with oil and gas discharge

Abstract

AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials AME 76:95-109 (2015) - DOI: https://doi.org/10.3354/ame01773 Bacterial communities in sediments of Lake Baikal from areas with oil and gas discharge Tamara I. Zemskaya*, Anna V. Lomakina, Elena V. Mamaeva, Alexandra S. Zakharenko, Tatyana V. Pogodaeva, Darya P. Petrova, Yuri P. Galachyants Limnological Institute, Siberian Branch of the Russian Academy of Sciences, Ulan-Batorskaya Street 3, 664033 Irkutsk, Russia *Corresponding author: tzema@lin.irk.ru ABSTRACT: Diversity and abundance of bacterial communities were investigated in methane hydrate- and oil-bearing sediments with different composition of pore waters at 6 sites in Lake Baikal, Siberia, using massively parallel sequencing (the Roche 454 platform). Sequences of Proteobacteria (17-48%), Actinobacteria (19-44%) and Cyanobacteria (17-48%) dominated the communities at all sites. Phylogenetic analysis of cyanobacterial sequences showed a large contribution of planktonic species and presence of uncultured lineages, whose representatives were detected in sediment communities of freshwater lakes. The ratio of different classes of Proteobacteria varied considerably: the Epsilon- and Delta- classes were the least represented, while the Alpha-, Beta- and Gamma- classes were the most represented. Some samples also showed a significant contribution of Bacteroidetes (7-13%), Chloroflexi (13%), candidate division OP10 and unclassified sequences (up to 16%). Other taxa made up less than 1%. Bacterial communities included a large number of unique phylotypes, whereas only few conventional phylotypes were detected. The phylotypes dominating the communities belonged to several taxa with cosmopolitan distributions. Their closest homologues involved liquid and gaseous hydrocarbons from deep sediments in biological cycles. Bacterial communities in Lake Baikal possessed moderate bacterial richness compared with other lake ecosystems. KEY WORDS: Lake Baikal · Sediments · Methane hydrates · Oil and methane seeps · Mud volcano · Bacterial communities · Pyrosequencing Full text in pdf format Supplementary material PreviousNextCite this article as: Zemskaya TI, Lomakina AV, Mamaeva EV, Zakharenko AS, Pogodaeva TV, Petrova DP, Galachyants YP (2015) Bacterial communities in sediments of Lake Baikal from areas with oil and gas discharge. Aquat Microb Ecol 76:95-109. https://doi.org/10.3354/ame01773 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in AME Vol. 76, No. 2. Online publication date: October 07, 2015 Print ISSN: 0948-3055; Online ISSN: 1616-1564 Copyright © 2015 Inter-Research.