Abstract
Traceability of seafood has become crucial with market globalization and consumer’s awareness. The present study used PCR-DGGE and 454 pyrosequencing to assess if bacterial communities fingerprint associated to seabass (Dicentrarchus labrax) skin mucus can be used to discriminate the geographic origin of fishes cultured in three semi-intensive fish farms. PCR-DGGE and pyrosequencing results were congruent and suggested that this molecular approach has the potential to trace fish farms with a spatial resolution <500 m. Pyrosequencing results provided a detailed insight into the bacterial community composition of seabass skin mucus and revealed the existence of a core of bacterial communities within family Pseudomonadaceae and Rhodobacteraceae. This approach also allowed to recognized key OTUs that are potentially relevant to discriminate the geographic origin of the fish being surveyed. Overall, the present study increased our knowledge on farmed seabass microbiome and demonstrated that specific and unique bacterial taxa can act as natural signatures that allow us to trace fish to its respective geographic origin. Our study provides valuable clues that should be more investigated in future studies as a way to fulfill current traceability needs in the global trade of seafood.
Highlights
Current needs on seafood traceability have driven relevant biotechnological breakthroughs that can have direct applications in the authentication or/and origin certification of seafood[3,9,10]
The present study aimed to evaluate if bacterial communities fingerprint of seabass skin mucus can be used to successfully discriminate the origin of specimens produced in three semi-intensive fish farms using a polymerase chain reaction (PCR)-Denaturing gradient gel electrophoresis (DGGE) and 454 pyrosequencing approach
These results support the non-metric multidimensional scaling (NMDS) representation which exhibits farm A as the most dissimilar in terms of bacterial communities displayed on the PCR-DGGE profile
Summary
Current needs on seafood traceability have driven relevant biotechnological breakthroughs that can have direct applications in the authentication or/and origin certification of seafood[3,9,10]. Generation Sequencing (NGS), such as 454 pyrosequencing technique, allows to understand in detail the microbial composition associated with a specific community and provides a simple and cost-effective mechanism for characterizing the composition of bacterial communities[19,20,21] This NGS method allows to recognize the microbial profile associated to each PCR-DGGE fingerprint and offers the possibility to identify taxonomically any given microbiological signature. While fish farms can promote their products based on safety and sustainable aquaculture practices, adding value to their products, consumers willing to pay more for such premium products may be offered a better warranty to verify the claims made by producers Another important aspect to take into account to achieve a suitable traceability methodology using the tools described above relies on the morphological structure of fish being surveyed. The present study aimed to evaluate if bacterial communities fingerprint of seabass skin mucus can be used to successfully discriminate the origin of specimens produced in three semi-intensive fish farms using a PCR-DGGE and 454 pyrosequencing approach
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
