Abstract
Escherichia coli has been the pioneering host for recombinant protein production, since the original recombinant DNA procedures were developed using its genetic material and infecting bacteriophages. As a consequence, and because of the accumulated know-how on E. coli genetics and physiology and the increasing number of tools for genetic engineering adapted to this bacterium, E. coli is the preferred host when attempting the production of a new protein.
Highlights
Escherichia coli has been the pioneering host for recombinant protein production, since the original recombinant DNA procedures were developed using its genetic material and infecting bacteriophages
Bacterial hosts others than E. coli are attracting attention as cell factories due to their metabolic diversity and biosynthetic potential derived from adaptation to extremely diverse environments
The implementation of lactic acid bacteria as a routine cell factory expands their applications from conventional food microbiology [18-21] to protein production and protein drug display and delivery [22-29], taking advantage of the generically recognized as safe (GRAS) features of this platform
Summary
Towards universal systems for recombinant gene expression. Microb Cell Fact 2010, 9:27. 2. 3. Ward OP: Production of recombinant proteins by filamentous fungi. 4. Arya R, Bhattacharya A, Saini KS: Dictyostelium discoideum–a promising expression system for the production of eukaryotic proteins. 6. Potvin G, Zhang Z: Strategies for high-level recombinant protein expression in transgenic microalgae: a review. 8. Porro D, Gasser B, Fossati T, Maurer M, Branduardi P, Sauer M, et al: Production of recombinant proteins and metabolites in yeasts: when are these systems better than bacterial production systems? 9. Corchero JL, Gasser B, Resina D, Smith W, Parrilli E, Vazquez F, et al: Unconventional microbial systems for the cost-efficient production of high-quality protein therapeutics. Decker EL, Reski R: Current achievements in the production of complex biopharmaceuticals with moss bioreactors. Gerngross TU: Advances in the production of human therapeutic proteins in yeasts and filamentous fungi. Specht E, Miyake-Stoner S, Mayfield S: Micro-algae come of age as a platform for recombinant protein production.
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