Bacterial cell division is involved in the damage of gram-negative bacteria on a nano-pillar titanium surface

Abstract

The role of bacterial cell division on the damage of adherent bacteria to titanium (Ti) nano-pillar cicada wing like surface was analyzed. Therefore nano-pillar Ti thin films were fabricated by glancing angle sputter deposition (GLAD) on silicon substrates. Gram-negative E. coli bacteria were allowed to adhere and to proliferate on these nanostructured samples for 3 h at 37 °C either under optimal cell growth conditions (brain heart infusion medium, BHI) or limited growth conditions (RPMI1640 medium). The bacteria adhered to the samples in both media. Compared to BHI medium the growth of E. coli in RPMI1640 medium was significantly inhibited. Concomitantly, the ratio of dead/living adherent bacteria on the nano-pillar surface was significantly decreased after the incubation period in RPMI1640. In addition, when the bacterial proliferation was biochemically halted using DL-serine-hydroxamate a comparable decrease in the ratio of dead/living adherent bacteria was also obtained in BHI medium. These results indicate that cell growth of adherent E. coli which is accompanied by cell elongations of the rod structure is involved in the damage induced by the titanium nano-pillar surface.