Bacterial Artificial Chromosome Transgenesis Through Pronuclear Injection of Fertilized Mouse Oocytes

Kristina Vintersten,A. Francis Stewart,Giuseppe Testa,Ronald Naumann,Konstantinos Anastassiadis

doi:10.1007/978-1-59745-570-1_5

Bacterial Artificial Chromosome Transgenesis Through Pronuclear Injection of Fertilized Mouse Oocytes

Kristina Vintersten, A. Francis Stewart + Show 3 more

https://doi.org/10.1007/978-1-59745-570-1_5

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Publication Date: Jan 1, 2008

Citations: 21

Affiliation: Mount Sinai Hospital, Lunenfeld-Tanenbaum Research Institute, TU Dresden, FIRC Institute of Molecular Oncology, Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Institut für Zellbiologie

#Bacterial Artificial Chromosomes #Bacterial Artificial Chromosome Transgenesis + Show 8 more

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Abstract

In the mouse, conventional transgenes often produced unpredictable results mainly because they were too small to recapitulate a natural gene context. Bacterial artificial chromosomes (BACs) are large enough to encompass the natural context of most mammalian genes and consequently deliver more reliable recapitulations of their endogenous counterparts. Furthermore, recombineering methods now make it easy to engineer precise changes in a BAC transgene. Consequently, BACs have become the preferred vehicle for mouse transgenesis. Here, we detail methods for BAC transgenesis through pronuclear injection of fertilized oocytes.

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