Bacterial anti-adhesion activity based on the electrochemical properties of polymethacrylates bearing ferrocenyl pendant groups

Abstract

Much current research is focused on preventing and controlling the natural process of colonization by marine organisms of surfaces submerged in seawater. Previously, the authors’ laboratory has reported the synthesis and the full physico-chemical characterization of homopolymers obtained from 1-ferrocenylmethyl methacrylate (FMMA), 2-(ferrocenylmethoxy)ethyl methacrylate (FMOEMA), and 3-(ferrocenylmethoxy)propyl methacrylate (FMOPMA). Here, the bacterial anti-adhesion activity of these homopolymers (pFMMA, pFMOEMA and pFMOPMA) is reported when stimulated in 96-well microplates containing a printed electrochemical cell in each well. Polymers were deposited on the printed carbon working electrode of each well in two columns each comprising eight wells. Their electrochemical anti-adhesion properties were evaluated by inoculating a marine biofilm forming bacterial strain, Pseudoalteramonas lipolytica, in each well and then applying recurrent scans for 15 h. The results revealed an intrinsic anti-adhesion activity of all the polymers. This activity was amplified by a factor of 10 when potential recurrent scans were applied.