Abstract
In vitro fertilization (IVF) is the most advanced technique of assisted reproductive where the sperm reach the eggs for fertilization in a laboratory tube. Seminal fluid often contains bacterial contaminants that decrease the quality and quantity of seminal fluids.
 Aim: In this study we investigated the presence of bacteria in semen before IVF procedure.
 Methodology: All samples were identified according to the morphological, biochemical, molecular, and antimicrobial tests.
 Results: Microbial species were detected in 28% of semen samples, while 72% did not show any bacterial growth. The prevalent bacterial isolates were Staphylococcus spp., Enterococcus spp., Catellicoccus spp., and Pseudomonas spp.
 Conclusion: The use of microbiological analysis to identify resistant bacterial isolates is considered an effective method to reduce infertility in men by changing the contaminated semen.
Highlights
Seminal fluid is an organic liquid that may contain spermatozoa
17 (28%) of the 60 semen specimens were positive for bacterial growth, while 43 (72%)
All of bacterial isolates could be confidently identified to six different species, SF1B showed 90% similarity with the type strain Staphylococcus spp., SF2B shared 90% similarities with Enterococcus faecalis, SF3B was similar to Pseudomonas spp. by 92% similarity for the, SF4B showed 86% similarity with the Catellicoccus spp., SF5B was 82% similar to Staphylococcus warneri, and SF6B was similar to Enterococcus hirae by 88% as shown in Table (2)
Summary
Seminal fluid is an organic liquid that may contain spermatozoa. It is secreted by the sexual glands of male or hermaphroditic animals and can fertilize female ovum. Seminal fluid contains various components besides spermatozoa: proteolytic and other enzymes as well as fructose. Bacterial assessment of seminal fluid in the weeks preceding In vitro fertilization (IVF) procedure is requested to avoid low sperm quality. The deficiency of seminal fluid accounts for 7% of Human infertility [2]. Bacteria isolated from this fluid account for a small proportion of the total microorganism number, they pose opportunistic behaviors that affect sperm quality, along with distortions (especially aberrant motility) loss of DNA integrity and deficient mitochondrial function [3,4]. Personal habits such as sexual promiscuity and poor hygiene, increase the bacterial isolates number and change the basic characteristics of semen
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