Bacterial Adherence Around Sutures of Different Material at Grafted Site: A Microbiological Analysis.

Lanka Mahesh,Sagrika Shukla,Anshi Jain,Juan Manuel Aragoneses,Manuel Fernández-Domínguez,José María Martínez González,José Luis Calvo-Guirado,Varun Raj Kumar

doi:10.3390/ma12182848

Abstract

Closure of the surgical incision has been the primary function of sutures since their introduction. However, whatever the type, they are known to carry bacteria, which can be a source of infection. Five types of surgical sutures, Gut, Silk, Vicryl, PTFE, and Polyamide, were selected and tested on their ability to carry aerobic and anaerobic bacteria and were rated on the basis of forming colony-forming units (CFUs). Aerobic bacteria grown around gut sutures showed minimum CFUs (≈30 × 104/suture). Though very less anaerobic bacteria growth was seen among all tested suture materials, it was maximum around Vicryl and polyamide sutures. Every suture material is capable, albeit not equally, of holding bacterial biofilm formation, which can be a source of surgical site infection.

Highlights

Since 3500 BC, sutures have been considered to be the most effective and useful method for the closure of surgical incisions and they have becomes an integral part of surgical procedures [1]
Aerobic bacteria grew around gut sutures (Figure 2a) and Results were expressed as the mean colony-forming units (CFUs) (± standard deviation) of total bacteria for both
Figure sutures.2. (a) Aerobic bacteria grown around gut sutures, (b) anaerobic bacteria grown around gut sutures

Summary

Introduction

Since 3500 BC, sutures have been considered to be the most effective and useful method for the closure of surgical incisions and they have becomes an integral part of surgical procedures [1]. There are various types of sutures, such as absorbable/non-absorbable, synthetic/natural, monofilament/braided, etc., but all are known to attract bacteria, which can be a source of infection at the surgical site [1]. During wound closure, procedure bacteria have the potential to move from the superficial skin surface into the deeper tissue layers carried by suture materials. Bacterial accumulation at the surgical site changes and creates a hypoxic environment within and around the wound as well as inhibiting the activity of fibroblasts, which result in delayed healing [3]. Suture contamination by bacterial accumulation further leads to wound decontamination. The process of wound decontamination is mediated by the inhibition

Methods

Results

Discussion

Conclusion