Abstract
The abundance and diversity of bacteria in two types of ponds were investigated by quantitative PCR and Illumina MiSeq sequencing. The results revealed that the abundance of bacterial 16S rRNA genes in D ponds (with grass carp fed sudan grass) was significantly lower than that in E ponds (with grass carp fed commercial feed). The microbial communities were dominated by Proteobacteria, Cyanobacteria, Bacteroidetes, and Actinobacteria in both E and D ponds, while the abundance of some genera was significantly different between the two types of ponds. Specifically, some potential pathogens such as Acinetobacter and Aeromonas were found to be significantly decreased, while some probiotics such as Comamonadaceae unclassified and Bacillales unclassified were significantly increased in D ponds. In addition, water quality of D ponds was better than that of E ponds. Temperature, dissolved oxygen and nutrients had significant influence on bacterial communities. The differences in bacterial community compositions between the two types of ponds could be partially explained by the different water conditions.
Highlights
High-throughput sequencing, such as in marine water[19], natural wetland[20], soil[21], drinking water[22], activated sludge[23] and hot spring[24]
We showed the vertical stratification of the 16S rRNA bacterial abundance in the water layers of two different types of aquaculture ponds
These results indicate that temperature may play an important role in the seasonal dynamics of bacterial abundance
Summary
High-throughput sequencing, such as in marine water[19], natural wetland[20], soil[21], drinking water[22], activated sludge[23] and hot spring[24]. These recent advances in DNA sequencing technologies allow us to develop a broader understanding of complex microbial communities affected by adding carbon sources into aquaculture system. We hypothesized that adding carbon sources into aquaculture system will improve water quality and change microbial communities in water column. The findings of this study will help to understand the impact of the additional carbon sources on microbial communities in aquaculture ponds, and evaluated the impact of adding carbon sources on culture environment
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