Bacteria inhibition of platelet concentrates with and without pre-centrifugation metronidazole incorporation on aggregatibacter actinomycetemcomitans

Abstract

Background: Local administration of antibiotics was one of many approaches for the management of localized periodontal infection with the advantages of smaller total dosage delivered into the pocket, avoiding systemic antibiotic side effects, and increasing the exposure of target microorganism to higher concentration. Incorporating antibiotics into platelet concentrates can be considered for local antibiotic administration.Method: The sample in this study used Aggregatibacter actinomycetemcomitans which was divided into 4 partitions on each plate. Each plate was applied with Platelets Rich Fibrin (PRF) and Injectable Platelet rich fibrin (I-PRF) incorporated with metronidazole pre-centrifugation and two negative controls (PRF and I-PRF without metronidazole). The observation was performed after 1 day and 7 days of incubation by measuring inhibition zone diameter using vernier caliper. The data were analyzed using two-way ANOVA followed by post-hoc LSD.Result: I-PRF with metronidazole had the highest bacteria inhibition compared with other groups (p<0,05) on incubation day 1 and 7; I-PRF and PRF with metronidazole showed higher bacteria inhibition compared with I-PRF and PRF without metronidazole (p<0,05).Conclusion: Aggregatibacter actinomycetemcomitans inhibition of PRF and I-PRF incorporated with metronidazole was greater compared to PRF and I-PRF without incorporating metronidazole, and A. actinomycetemcomitans inhibition of I-PRF incorporated with metronidazole was greater than PRF incorporated with metronidazole.